2011—2013年河南省犬细小病毒分子流行病学调查与分析

doi:10.11843/j.issn.0366-6964.2014.10.013

Abstract

Abstract:

In order to investigate the prevalence and the molecular evolutionary characteristics of canine parvovirus (CPV) infection in Henan province during 2011-2013，573 suspected CPV samples were collected from 10 different areas，such as Zhengzhou，Luoyang，Kaifeng，Xinyang，etc.Partial of the VP2 fragments (462-1 023 bp) of CPV were amplified by national standard method (NSM).Based on the sequenced results and phylogenetic tree analysis，the representative strains were selected and complete VP2 genes were cloned，sequenced and analyzed.The results showed that the positive rate was 88.48% by NSM，and the positive strains were divided into 7 branches.According to the VP2 gene characteristics，6 branches were CPV-2a type，and 1 branch of CPV was CPV-2b type.Sequence analysis for VP2 gene between CPV isolates and 30 CPV representative strains accessed in GenBank showed that the homology of nucleotides and amino acids were 98.4%-99.9% and 97.6%-100.0%，respectively.Phylogenetic analysis indicated that the 7 CPV isolations were divided into two groups，only one strain (No.KJ438801) was far from the other 6 isolates.The comparison of amino acid sequence showed that 12 mutated sites were found，of which，the 267，297，324，440，555 amino acid sites were easiest to mutate，and there were at least 2-3 sites of amino acid mutated in each strain.These results indicated that the CPV-2a and CPV-2b were coexisted and the CPV-2a was the primary pathogen in Henan province.More importantly，the VP2 gene showed multiple variations in different strains.The results of this study provide a scientific basis for improving the vaccine design and the control of CPV.

CLC Number:

S852.659.2

DING Ke，YU Zu-hua，PENG Chun-ping，ZHAO Zhan-qin，HE Lei，JIA Yan-yan，ZHANG Chun-jie，CHENG Xiang-chao，XIA Xian-zhu. Molecular Epidemiological Investigation of Canine Parvovirus Isolates from Henan Province during 2011-2013[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(10): 1671-1678.

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Molecular Epidemiological Investigation of Canine Parvovirus Isolates from Henan Province during 2011-2013

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